# A simple BASIC - program

This short program allows the simulation of essential patterns in development: the generation of gradients, of periodic structures, of polar patterns that oscillate and of initiation of leaves in a helical arrangement (phyllotaxis). It is written in BASIC The program can be changed and recompiled with compilers that are freely available on the web. Details are given here. For an executable file in a compressed and the source code form click here. The resulting screens are given at the end of this page. A short introduction how to write such a program is given in a commented PowerPoint presentation [PPT]

'------------------------------------------------------------

' A simple program to simulate biological pattern formation

' (C) Hans Meinhardt, MPI Tuebingen

DEFDBL A-G

DEFDBL O-Z

DEFINT H-N

ON ERROR GOTO checkerror

' A simple program to simulate biological pattern formation

' (C) Hans Meinhardt, MPI Tuebingen

' The program is written in BASIC. Convenient compilers are

' Freebasic for Windows or Unix, PowerBasic 3.5 for DOS

' Microsoft QB 4.5, or QBX

' i = 1 ... n= Number of cell

imax = 640: DIM ax(imax), bx(imax), cx(imax), s(imax)

simtyp$ = "p"' First simulation: periodic pattern

start:

SELECT CASE simtyp$

CASE "p": text$ = "Periodic Pattern"

KT = 100 'Number of displays

KP = 20 ' 'number of iterations between the displays

'it*ip = number of iterations in total

n = 40' number of cells

DA = .01' Diffusion of the activator

RA = .02' Removal rate of the activator

BA = .001 ' Activator-independent activator production rate

DB = .4' Diffusion of the inhibitor

RB = .03' Removal rate of the inhibitor

RC = 0' only Activator-Inhibitor system, Substance c not in use

fa = 60: fb = 60: fc = 0' Scaling factors for display

igraph = 1: boundary$ = "tight"

CASE "g": text$ = "Graded Distribution"

KT = 50: n = 12: KP = 200

DA = .004: RA = .0015: BA = .0001: DB = .4: RB = .0025: RC = 0

igraph = 1: boundary$ = "tight": fa = 60: fb = 60: fc = 0

CASE "o": text$ = "Pattern destabilizes itself: polar oscillations"

KT = 200: KP = 500: n = 12: DA = .003: RA = .002: BA = .05

DB = .4: RB = .003: DC = .001: RC = .0001:

igraph = 1: fa = 40: fb = 50: fc = 50: boundary$ = "tight"

CASE "h": text$ = "Pattern destabilizes itself: helical phyllotaxis"

KT = 144: KP = 400: n = 40: DA = .005: RA = .004: BA = .05

DB = .4: RB = .006: DC = .003: RC = .0002:

igraph = 2: fa = 40: fb = 50: fc = 50' Graph-type and Scaling

boundary$ = "cyclic"

CASE ELSE: GOTO TheEND ' each other key: the end

END SELECT

REM ----------- Initial conditions --------------------------

FOR i = 1 TO n

s(i) = RA * (.99 + .02 * RND)'"Source density" = Production of the

'activator, proportional to the decay rate +- 2% fluctuation

ax(i) = 1'general initial activator concentration

bx(i) = 1'general initial inhibitor concentration

cx(i) = 0: NEXT

SCREEN 12: t = TIMER: CLS 'Initialization of graphic and timing

WINDOW (1, 1)-(640, 480) ' coordinate system

LINE (1, 1)-(640, 480), 15, BF 'background white

LOCATE 30, 1:

PRINT "green = Activator, red = Inhibitor, blue = Source density; any key: stop";

LOCATE 1, (80 - LEN(text$)) / 2: PRINT text$

continuo: ' if calculation is continued...

DeltaWi = 6.28 / n: yzyl = 30: radius = 100

perspective = .15' for cylinder of phyllotaxis

idx = 600 / n: fs = 350 / RA 'Pixel-size of a cell

FOR itot = 0 TO KT' total numberplots

FOR iprint% = 1 TO KP' Calculations between plots

SELECT CASE boundary$

CASE "cyclic"

al = ax(n): bl = bx(n): cl = cx(n)'al is the concentration in the cell

'left of the actual cell. Left-most cell = last cell = cyclic

ax(n + 1) = ax(1)' Concentration in a virtual cell on the right

bx(n + 1) = bx(1)' equal concentration of the left-most cell

cx(n + 1) = cx(1)' for all substances

CASE "tight"

al = ax(1): bl = bx(1): cl = cx(1)'al is the concentration in the cell

'left of the actual cell. Left-most cell = virtual cell with the same

'concentration

ax(n + 1) = ax(n)' concentration in a virtual cell to the right of the

bx(n + 1) = bx(n)' right-most cell is equal to the concentration in the

cx(n + 1) = cx(n)' right-most cell, this for all substances

END SELECT

REM ---------- Reactions ------

FOR i = 1 TO n' i = current cell, n = right cell

a = ax(i) 'local activator-concentration

b = bx(i) 'local inhibitor1-concentration

c = cx(i) 'local inhibitor2-concentration

'Calculation of a new activator and inhibitor concentration in cell i

Diffactivator = DA * ((al - a) + (ax(i + 1) - a)) 'Exchange with neighbor

ax(i) = a + s(i) * (a * a + BA) / (b + c) - RA * a + Diffactivator

bx(i) = b + s(i) * a * a - RB * b + DB * ((bl - b) + (bx(i + 1) - b))

IF RC > 0 THEN ' c only in oscillating patterns and phyllotaxis

cx(i) = c + RC * a - RC * c + DC * ((cl - c) + (cx(i + 1) - c))

END IF

al = a: bl = b: cl = c'The not yet changed concentrations

'are used as left cell concentrations in the subsequent cell

NEXT i

NEXT iprint%

REM ----------------Plot -------------

SELECT CASE igraph

CASE 1' Each cell is a rectangle

LINE (20, 45)-(620, 50), 1, BF

x1 = 20' Position of the first rectangle

FOR i = 1 TO n

x2 = x1 + idx

afl = 51 + ax(i) * fa: LINE (x1, 51)-(x2, afl), 2, BF'green=activator

LINE (x1, afl)-(x2, 450), 15, BF 'remaining part white: erase old plot

bfl = 51 + bx(i) * fb: LINE (x1, bfl)-(x2, bfl + 10), 12, BF

sfl = 51 + s(i) * fs: LINE (x1, sfl)-(x2, sfl + 3), 1, BF

IF RC > 0 THEN

cfl = 51 + cx(i) * fc: LINE (x1, cfl)-(x2, cfl + 3), 13, BF 'second inhibitor

END IF

x1 = x2: NEXT

DO UNTIL TIMER - t > .1: LOOP: t = TIMER'slow down if computer is too fast

CASE 2 ' transparent cylinder for phyllotaxis

LOCATE 30, 1: PRINT "black/grey = high activator concentration, y-coordinate=time, any key=stop";

wi1 = 0: n21 = n / 2 + 1 'front or back of the cylinder

yzyl = yzyl + 3'Next plot one pixel row higher

FOR ix% = 1 TO n'Black if above a threshold

wi2 = wi1 + DeltaWi

ico = 0: IF ix% < n21 THEN ico = 7 'Cells are gray if seen from inside

IF ax(ix%) > 1 THEN

CIRCLE (320, yzyl), radius, ico, wi1, wi2, perspective'Several lines

CIRCLE (320, yzyl + 1), radius, ico, wi1, wi2, perspective'on top of

CIRCLE (320, yzyl + 2), radius, ico, wi1, wi2, perspective'each other

END IF

wi1 = wi2: NEXT ix%

IF icount = 0 THEN CIRCLE (320, yzyl), radius, 10, , , perspective

icount = icount + 1 'green circle for clarity

IF icount = 3 THEN icount = 0

END SELECT

IF INKEY$ > "" THEN EXIT FOR 'any key -> exit

NEXT itot

LOCATE 30, 1: PRINT "c = continue; s = start again; n = new type of ";

PRINT "simulation, ... other keys = end";

a$ = "": DO UNTIL a$ > "": a$ = INKEY$: LOOP' wait for key

SELECT CASE a$

CASE "c", "w": GOTO continuo

CASE "s": GOTO start

CASE "n": LOCATE 30, 1:

PRINT "p = Periodic; g= gradient, o = oscillating polar, h = helical,other key = end";

simtyp$ = "": DO UNTIL simtyp$ > "": simtyp$ = INKEY$: LOOP' wait for key

GOTO start

END SELECT

TheEND:

LOCATE 30, 1: t = TIMER

PRINT ".... a program for simulation of biol. pattern formation;(c) Hans Meinhardt";

DO UNTIL TIMER - t > 3: LOOP

END

checkerror:

IF ERR > 0 THEN PRINT "There was an error, code "; ERR, : SLEEP

RESUME NEXT

'-------------------End of the Program-------------------------------

Running this program lead to the following screens:

- Periodic pattern
- Gradients
- Oscillations of polar patterns
- Phyllotaxis

[Next: Fading of competence, suppression of supernumerary organizers and the generation tissue polarity]

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Feedback to: mailto:hans.meinhardt@tuebingen.mpg.de