The Genome Center Facility and the Department of Molecular Biology under the leadership of Detlef Weigel introduced next-generation DNA sequencing to our institute already in 2007. Today, sequencing-based approaches are crucial for the large majority of our research groups. These include de novo genome assemblies, variant discovery, metagenomics, chromatin analysis, full-length and short read transcriptomics, small RNA analysis and single-cell applications for organisms as diverse as vertebrates, insects, worms, plants, brown algae, fungi, ciliates and other microbes.
The Genome Center hosts a Sequel II instrument from Pacific Biosciences for high-fidelity single-molecule real-time (SMRT) sequencing of DNA fragments with many thousand nucleotides in length. The facility also operates a NextSeq 2000 platform from Illumina routinely generating paired-end short-read data (up to 2 x 300 nucleotides) from small DNA fragments in the order of 360 gigabases per run. Finally, an Illumina MiSeq sequencer is available for small-scale projects.
The Genome Center assures fast, reliable and high-quality sequencing services and supports the development of new application protocols. The facility trains internal and external scientists in preparing libraries, assists with technical equipment for library preparation and quality control, and provides dedicated lab space.
- Sequel II, Pacific Biosciences
- NextSeq 2000, Illumina
- MiSeq, Illumina
- Chromium, 10x Genomics
- Femto Pulse, Agilent Technologies
- Bioanalyzer 2100, Agilent Technologies
- Sage ELF, Sage Science
- BluePippin, Sage Science
- CHEF-DR III, BioRad
- Megaruptor 2, Diagenode
- Covaris S2, KBiosciences
- Qubit 2.0 Fluorimeter, Invitrogen
- Viaflo 96, Integra