Equipment

Transmission Electron Microscopes (TEM)

  • 200 kV Talos Arctica with a Falcon III and Ceta cameras, Volta phase plate
  • 120kV Tecnai G2 Sprit BioTWIN with a TVIPS F416 camera, +/-70° tomography holder and Gatan cryo-holder

Scanning Electron Microscope (SEM)

  • Hitachi Regulus SU8230

Sample Preparation

  • High Pressure Freezer, Wohlwend Compact 03 for the cryo fixation of biological samples
  • Freeze Substitution, Leica AFM2 for the dehydration and embedding of cryo-fixed samples
  • Leica EM GP plunge freezer
  • Thermo Fisher Vitrobot Mark IV plunge freezer
  • Balzers BAF 300 for freeze fracture and metal shadowing of samples
  • Safematic CCU-010 sputter coater for SEM sample preparation
  • Polaron E3100 critical point dryer for the gentle dehydration of SEM samples
  • Leica CPD300 critical point dryer
  • RMC QG-3000EC automated grid stainer

Sample Sectioning

  • Leica UC7 with FC7 for cryo-sectioning
  • Leica Ultracut UCT
  • Reichert Ultracut S with Reichert FC S cryo-box for cryo-sectioning
  • Reichert Ultracut E
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