Equipment
Transmission Electron Microscopes (TEM)
- 200 kV Talos Arctica with a Falcon III and Ceta cameras, Volta phase plate
- 120kV Tecnai G2 Sprit BioTWIN with a TVIPS F416 camera, +/-70° tomography holder and Gatan cryo-holder
Scanning Electron Microscope (SEM)
Sample Preparation
- High Pressure Freezer, Wohlwend Compact 03 for the cryo fixation of biological samples
- Freeze Substitution, Leica AFM2 for the dehydration and embedding of cryo-fixed samples
- Leica EM GP plunge freezer
- Thermo Fisher Vitrobot Mark IV plunge freezer
- Balzers BAF 300 for freeze fracture and metal shadowing of samples
- Safematic CCU-010 sputter coater for SEM sample preparation
- Polaron E3100 critical point dryer for the gentle dehydration of SEM samples
- Leica CPD300 critical point dryer
- RMC QG-3000EC automated grid stainer
Sample Sectioning
- Leica UC7 with FC7 for cryo-sectioning
- Leica Ultracut UCT
- Reichert Ultracut S with Reichert FC S cryo-box for cryo-sectioning
- Reichert Ultracut E