Sample preparation techniques

  • Negative staining
  • Plunge freezing
  • Chemical fixation
  • High-pressure freezing of cells, organisms and tissues
  • Freeze substitution
  • Resin embedding
  • Ultramicrotomy
  • Cryo-ultramicrotomy for the Tokuyasu technique
  • On-section immuno-labelling
  • Shadowing
  • Critical point drying
  • Sputter-coating
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