Equipment
BioOptics Facility
Confocal Microscopes
Zeiss LSM 980 NLO Airyscan
Confocal laser scanning microscope:
- 8 cw laser lines
- Quasar unit for normal and hyperspectral imaging in the visible range
- 2 additional channels for NIR imaging
- Airyscan 2 unit for superresolution imaging
- pulsed and tunable infrared laser and non-descanned detectors for two-photon imaging
- fully motorised inverted stand
- camera
- incubation chamber
Nikon Eclipse Ti2-E with Crest X-Light V3
Spinning disc confocal microscope:
- 6 cw laser lines
- fully motorised inverted stand
- 2 cameras
- small incubation chamber (heating/ cooling) with full gas control
- Mightex Polygon DMD for photomanipulation
Leica TCS SP8
Confocal laser scanning microscope:
- fully motorised upright stand
- 4 cw laser lines
- 3 hybrid detectors and 1 PMT
Widefield Microscopes
Zeiss AxioImager
Upright widefield microscope:
- partially motorised stand
- sensitive sCMOS camera for fluorescence imaging
- high resolution CCD colour camera
- large set of fluorescence filters
- broad range of transmitted light modalities
Acquifer Imaging Machine
Inverted widefield microscope:
- fully motorised and programmable stand
- built-in incubation with high thermal stability
- static sample holder (the optics move underneath), ideal for delicate live samples
- fast switching LEDs for transmitted light and fluorescence illumination
- sensitive sCMOS camera
- 4 fluorescent channels and brightfield
Zeiss AxioZoom
Upright widefield microscope:
- fully motorised stand
- zoom optics
- optics for large field of view at high resolution
- highly sensitive and very fast sCMOS camera for fluorescence
- high resolution CCD colour camera
- large selection of fluorescent filters and contrasting options.
Sorting
Becton Dickinson FACS Melody
fluorescence based cell counter/ sorter:
- 3 lasers
- 4 detection channels
- cells can be sorted into tubes or multiwell plates
- simple and easy to use
- biosafety cabinet
Sartorius CellCelector
Image-based sorting microscope:
- inverted and fully motorised microscope in a biosafety cabinet
- 4 fluorescent channels, brightfield and phase contrast
- automated, semi-automated and manual workflows
- for objects 20µm - 220µm diameter with max. 2.5 picking events per minute
- source vessels: multiwell plates, chambered slides, 35mm dishes, nanowell arrays
- deposition vessels: PCR microtubes, multiwell plates, dishes on a heated/ cooled deck
Other Equipment
We also host microscopes equipped for reflective imaging and epi-darkfield, with camera lucida, with dipping objectives, etc. We have incubation options for all our inverted systems to enable long term live imaging. There is also a workstation available for demanding image analysis and processing.